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ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+

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ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+

Auteurs : RBID : Pascal:14-0090287

Descripteurs français

Pascal (Inist)
- Dispositif photoélectrochimique, Détecteur, Détection, Contamination.

English descriptors

KwdEn :
- Contamination, Detection, Detector, Photoelectrochemical device.

Abstract

Lead contamination is now widespread, and exposure to lead may cause adverse effects on human beings. In this study, a photoelectrochemical sensor based on flower-like ZnO nanostructures was developed for Pb²⁺ detection, using a Pb²⁺-dependent DNAzyme as the recognition unit and a double-strand DNA intercalator, Ru(bpy)₂(dppz)²⁺ (bpy=2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c] phenazine) as the photoelectrochemical signal reporter. The ZnO nanoflower was fabricated on an indium tin oxide (ITO) electrode by the convenient hydrothermal decomposition method. The morphology and photoelectrochemical property of the ZnO nanoflowers were characterized by SEM, XRD and photocurrent measurements. DNAzyme-substrate duplex was assembled on an ITO/ZnO electrode through electrostatic adsorption. In the presence of Pb²⁺, RNA-cleavage activity of the DNAzyme was activated and its substrate strand was cleaved, resulting in the release of Ru(bpy)₂(dppz)²⁺ from the DNA film and the concomitant photocurrent decrease. The detection principle was verified by fluorescence measurements. Under the optimized conditions, a linear relationship between photocurrent and Pb²⁺ concentration was obtained over the range of 0.5-20 nM, with a detection limit of 0.1 nM. Interference from other common metal ions was found negligible. Applicability of the sensor was demonstrated by analyzing lead level in human serum and Pb²⁺ spiked water samples. This facile and economical sensor system showed high sensitivity and selectivity, thus can be potentially applied for on-site monitoring of lead contaminant.

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<author><name>BINTIAN ZHANG</name>
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<s2>Fuzhou, Fujian 350002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Fuzhou, Fujian 350002</wicri:noRegion>
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<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Key Laboratory of Design and Assembly of Functional Nanostructures, Chinese Academy of Sciences</s1>
<s2>Fuzhou, Fujian 350002</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Fuzhou, Fujian 350002</wicri:noRegion>
</affiliation>
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<author><name>LILI LU</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences</s1>
<s2>Fuzhou, Fujian 350002</s2>
<s3>CHN</s3>
<sZ>2 aut.</sZ>
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<country>République populaire de Chine</country>
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<author><name>QICHANG HU</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Key Laboratory of Optoelectronic Materials Chemistry and Physical Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences</s1>
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<author><name>FENG HUANG</name>
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<author><name>ZHANG LIN</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Structures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences</s1>
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<front><div type="abstract" xml:lang="en">Lead contamination is now widespread, and exposure to lead may cause adverse effects on human beings. In this study, a photoelectrochemical sensor based on flower-like ZnO nanostructures was developed for Pb<sup>2+</sup>
 detection, using a Pb<sup>2+</sup>
-dependent DNAzyme as the recognition unit and a double-strand DNA intercalator, Ru(bpy)<sub>2</sub>
(dppz)<sup>2+</sup>
 (bpy=2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c] phenazine) as the photoelectrochemical signal reporter. The ZnO nanoflower was fabricated on an indium tin oxide (ITO) electrode by the convenient hydrothermal decomposition method. The morphology and photoelectrochemical property of the ZnO nanoflowers were characterized by SEM, XRD and photocurrent measurements. DNAzyme-substrate duplex was assembled on an ITO/ZnO electrode through electrostatic adsorption. In the presence of Pb<sup>2+</sup>
, RNA-cleavage activity of the DNAzyme was activated and its substrate strand was cleaved, resulting in the release of Ru(bpy)<sub>2</sub>
(dppz)<sup>2+</sup>
 from the DNA film and the concomitant photocurrent decrease. The detection principle was verified by fluorescence measurements. Under the optimized conditions, a linear relationship between photocurrent and Pb<sup>2+</sup>
 concentration was obtained over the range of 0.5-20 nM, with a detection limit of 0.1 nM. Interference from other common metal ions was found negligible. Applicability of the sensor was demonstrated by analyzing lead level in human serum and Pb<sup>2+</sup>
 spiked water samples. This facile and economical sensor system showed high sensitivity and selectivity, thus can be potentially applied for on-site monitoring of lead contaminant.</div>
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 detection, using a Pb<sup>2+</sup>
-dependent DNAzyme as the recognition unit and a double-strand DNA intercalator, Ru(bpy)<sub>2</sub>
(dppz)<sup>2+</sup>
 (bpy=2,2'-bipyridine, dppz=dipyrido[3,2-a:2',3'-c] phenazine) as the photoelectrochemical signal reporter. The ZnO nanoflower was fabricated on an indium tin oxide (ITO) electrode by the convenient hydrothermal decomposition method. The morphology and photoelectrochemical property of the ZnO nanoflowers were characterized by SEM, XRD and photocurrent measurements. DNAzyme-substrate duplex was assembled on an ITO/ZnO electrode through electrostatic adsorption. In the presence of Pb<sup>2+</sup>
, RNA-cleavage activity of the DNAzyme was activated and its substrate strand was cleaved, resulting in the release of Ru(bpy)<sub>2</sub>
(dppz)<sup>2+</sup>
 from the DNA film and the concomitant photocurrent decrease. The detection principle was verified by fluorescence measurements. Under the optimized conditions, a linear relationship between photocurrent and Pb<sup>2+</sup>
 concentration was obtained over the range of 0.5-20 nM, with a detection limit of 0.1 nM. Interference from other common metal ions was found negligible. Applicability of the sensor was demonstrated by analyzing lead level in human serum and Pb<sup>2+</sup>
 spiked water samples. This facile and economical sensor system showed high sensitivity and selectivity, thus can be potentially applied for on-site monitoring of lead contaminant.</s0>
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ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+

ZnO nanoflower-based photoelectrochemical DNAzyme sensor for the detection of Pb2+

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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